Prevalence of Penicillium chrysogenum, its Qualitative, Quantitative Determination and Antibacterial Activity in Indian Soil
نویسندگان
چکیده
منابع مشابه
Penicillin acyltransferase in Penicillium chrysogenum.
Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase was purified threefold by adsorption on calcium phosphate gel at pH 6 and elutio...
متن کاملIdentification and Expression of Genes Involved in the Biosynthesis of Penicillin and Its Detection by HPLC in Penicillium chrysogenum
In this study, after identification of genes involved in biosynthesis of penicillin, we evaluated the expression of pcbAB and pcbC genes in P. Chrysogenum. A quantitative PCR (qPCR) approach was used to determine how these genes were expressed in different time courses. In addition, the produced penicillin content was measured using HPLC. qPCR analysis of mRNAs extracted from P. chrysogenum i...
متن کاملQualitative and Quantitative Approaches for Determination of Hemolyzed Serum
Abstract Background and Objective: Prevention of medical laboratory errors is a major goal of quality control programs. Hemolyzed specimen is one of the common issues causing medical laboratory errors. Apart from the visual assessment, the measurement of serum hemoglobin concentration can be another method to evaluate the intensity of hemolysis. We aimed to assess hemolyzed serum s...
متن کاملThe lactic dehydrogenase of Penicillium chrysogenum.
A soluble, cytochrome linked flavoprotein which oxidizes lactic acid to pyruvic acid has been isolated from Penicillium chrysogenum, strain NRRL 1951 -B25. The reduction of cytochrome c by a flavin enzyme has precedence in the work of Horecker and Heppel (1949) and Mlorell (1952) who have shown that xanthine oxidase can reduce cytochrome c. Morell reported the enzyme to be inhibited strongly by...
متن کاملBallistic disruption of Penicillium chrysogenum cells.
The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in...
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ژورنال
عنوان ژورنال: Asian Journal of Plant Sciences
سال: 2005
ISSN: 1682-3974
DOI: 10.3923/ajps.2005.429.434